In this study reverse transcription coupled with the polymerase chain reaction ( RT PCR) has been established to detect hog cholera virus ( HCV). 本研究建立了反转录聚合酶链反应(RTPCR)技术检测猪瘟病毒(HogCholeraVirus,HCV)方法。
[ Conclusion] Intramuscular cholera vaccine has good immunological reaction but requires a high dosage; 结论肌内注射霍乱疫苗产生良好的保护效果时需要较高剂量;
Construction of a mutant A subunit of cholera toxin elicited by polymerase chain reaction for site directed mutagenesis 用PCR体外定点突变技术诱导霍乱毒素A亚基突变体的构建